1UW9

L290F-A222T chlamydomonas Rubisco mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Collection date	2001-02-23
Spacegroup name	P 1 21 1
Unit cell lengths	126.073, 178.196, 120.463
Unit cell angles	90.00, 120.35, 90.00

Refinement procedure

Resolution	20.000 - 2.050
R-factor	0.1569
Rwork	0.157
R-free	0.19421
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gk8
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.150	0.490
Number of reflections	295370
<I/σ(I)>	8.9	1.8
Completeness [%]	88.9	83.5
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	291	0 MM HEPES PH 7.5, 8-12% PEG 4 50 MM NAHCO3, 5 MM MGCL2, 50 UM 2-CABP, 18 DEG C, 10-15 MG PROTEIN