1UVI
The structural basis for RNA specificity and Ca2 inhibition of an RNA-dependent RNA polymerase phi6p2 with 6nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2002-01-15 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.130, 93.711, 140.741 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.200* |
R-factor | 0.23 |
Rwork | 0.230 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hhs |
RMSD bond length | 0.011 |
RMSD bond angle | 23.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.126 | 0.818 |
Total number of observations | 1522219 * | |
Number of reflections | 142686 * | |
<I/σ(I)> | 13.3 | 1.6 |
Completeness [%] | 97.8 | 83.7 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | other * | 7.3 | Butcher, S.J., (2000) Acta Crystallogr.,Sect.D, D56, 1473. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10 (%) | |
3 | 1 | reservoir | MES | 0.1 (M) | pH6.7 |