1UV7

periplasmic domain of EpsM from Vibrio cholerae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-12
Detector	ADSC CCD
Wavelength(s)	0.9791,0.9795,0.9686
Spacegroup name	P 32 2 1
Unit cell lengths	52.879, 52.879, 112.484
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
Rwork	0.196
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	2.302
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.290	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.047	0.627
Number of reflections	20676
<I/σ(I)>	18	2.6
Completeness [%]	99.7	93.4
Redundancy	6.61	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	6-12MG/ML PROTEIN IN 20MM TRIS PH8, 150MM NACL, 1MM TCEP; RESERVOIR: 2.4-3.0M SODIUM MALONATE, 100MM TRIS PH~8; CRYSTALLISATION: 1.5MKL PROTEIN + 1.5MKL RESERVIOR, 4C, pH 8.00