Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.346, 60.760, 78.245 |
| Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 23.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.323 |
| Total number of observations | 122164 * | |
| Number of reflections | 42990 | 4166 * |
| <I/σ(I)> | 7.7 | 2.3 |
| Completeness [%] | 96.5 | 96.5 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 20% POLYETHYLENEGLYCOL 4000, IMIDAZOLE/MALATE 0.2M PH 6.0, 5% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.4 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH8 |
| 3 | 1 | reservoir | imidazole malate | 0.2 (M) | pH6.0 |
| 4 | 1 | reservoir | PEG4000 | 15-20 (%(w/v)) | |
| 5 | 1 | reservoir | glycerol | 5 (%) |
