1UUP

Crystal Structure of A Dimeric Form of Streptococcal Pyrogenic Exotoxin A (SpeA1).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	53.300, 127.200, 148.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.2152
Rwork	0.215
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b1z
RMSD bond length	0.007
RMSD bond angle	1.370
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.600
Rmerge	0.129
Number of reflections	31727
<I/σ(I)>	16.6
Completeness [%]	89.8
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.50