1UTY

Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-15
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	102.296, 102.296, 77.915
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.214
Rwork	0.211
R-free	0.26800
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.265
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.059	0.365
Number of reflections	18244
<I/σ(I)>	34.57	6.6
Completeness [%]	99.9	99.6
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20% JEFFAMINE M-600 PH 7.6, 0.65 M NACL, 10 MM SODIUM PHOSPHATE