1USO
DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-15 |
| Detector | RIGAKU IMAGE PLATE RAXIS-V |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.430, 48.687, 94.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.250 - 1.300 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1usm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.037 | 0.083 |
| Number of reflections | 34147 | |
| <I/σ(I)> | 29.35 | 17 |
| Completeness [%] | 94.0 | 95 |
| Redundancy | 4.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | VAPOR DIFFUSION METHOD, RESERVOIR SOLUTION 15% PEG8000 AND 0.2M AMMONIUM SULFATE. CRYOPROTECTANT 22.5% GLYCEROL, 15% PEG8000 AND 0.2M AMMONIUM SULFATE, pH 6.00 |
