1USM

DCOH, A BIFUNCTIONAL PROTEIN-BINDING TRANSCRIPTIONAL COACTIVATOR, PRO9LEU MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-15
Detector	RIGAKU IMAGE PLATE RAXIS-V
Spacegroup name	I 4 2 2
Unit cell lengths	55.625, 55.625, 125.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.770 - 1.200
R-factor	0.215
Rwork	0.215
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dco
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.034	0.146
Number of reflections	30991
<I/σ(I)>	41.4	11.7
Completeness [%]	98.7	99.5
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.3		MICROBATCH METHOD UNDER OIL BY CRYSTALLIZATION ROBOT TERA. 25.6 MG/ML OF PROTEIN SOLUTION WAS MIXED WITH 3.15M SODIUM FORMATE AND 0.1M SODIUM ACETATE BUFFER, PH 4.0. FINAL PH WAS 5.3. CRYOPROTECTANT CONTENT WAS: 25% GLYCEROL, 2.0M SODIUM FORMATE, AND 0.6M SODIUM ACETATE BUFFER, PH 4.0.