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human VASP tetramerisation domain L352M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Collection date | 2002-05-15 |
Spacegroup name | I 4 |
Unit cell lengths | 29.648, 29.648, 100.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.212 |
Rwork | 0.211 |
R-free | 0.22400 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.035 | 0.309 |
Number of reflections | 54657 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 25% PEG MME550, 0.2M NA-CITRATE, 0.1M TRIS PH8.5, pH 8.50 |