1US8
The Rad50 signature motif: essential to ATP binding and biological function
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 294 |
Collection date | 2001-11-15 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.541, 67.851, 69.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 2.100 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f2t |
RMSD bond length | 0.005 |
RMSD bond angle | 1.080 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.140 |
High resolution limit [Å] | 2.100 | 2.050 |
Rmerge | 0.057 | 0.279 |
Total number of observations | 200189 * | |
Number of reflections | 25989 | |
<I/σ(I)> | 10 | 3 |
Completeness [%] | 99.2 | 98.9 * |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 * | 20 % PEG8000, 0.1 M MES PH 6.0, 0.2 M CALCIUM ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4 (mg/ml) | |
10 | 1 | reservoir | calcium acetate | 0.2 (M) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.0 |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | EDTA | 0.5 (mM) | |
6 | 1 | drop | ATPgammaS | 2.5 (mM) | |
7 | 1 | drop | 10 (mM) | ||
8 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
9 | 1 | reservoir | MES | 0.1 (M) | pH6.0 |