1US0
Human Aldose Reductase in complex with NADP+ and the inhibitor IDD594 at 0.66 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-19 |
| Detector | ANL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.280, 66.590, 47.260 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.660 |
| R-factor | 0.0936 |
| R-free | 0.10320 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.680 |
| High resolution limit [Å] | 0.660 | 0.660 |
| Rmerge | 0.029 | 0.279 |
| Number of reflections | 511265 | |
| <I/σ(I)> | 14.5 | 2.4 |
| Completeness [%] | 89.1 | 75.4 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | AS DESCRIBED IN LAMOUR ET AL. (1999) ACTA CRYSTALL. SECTION D 55:721-723, pH 5.00 |
