1URT

MURINE CARBONIC ANHYDRASE V

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-06-02
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	101.090, 67.520, 48.860
Unit cell angles	90.00, 107.60, 90.00

Refinement procedure

Resolution	6.500 - 2.800
R-factor	0.137
Rwork	0.137
R-free	0.24100
RMSD bond length	0.010
RMSD bond angle	25.600 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.088
Total number of observations	19846 *
Number of reflections	6760 *
Completeness [%]	87.0 *
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop	sucrose monolaurate	2.0 (mM)
4	1	drop	PEG8000	16-12 (%)
5	1	drop	sodium acetate	0.2 (M)
6	1	drop	sodium cacodylate	0.1 (M)
7	1	reservoir	PEG8000	16-12 (%)
8	1	reservoir	sodium acetate	0.2 (M)
9	1	reservoir	sodium cacodylate	0.1 (M)