1URN

U1A MUTANT/RNA COMPLEX + GLYCEROL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	0.882
Spacegroup name	P 65 2 2
Unit cell lengths	97.000, 97.000, 255.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.920
R-factor	0.207 *
Rwork	0.207
R-free	0.24200 *
RMSD bond length	0.010
RMSD bond angle	0.035
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.500 *
High resolution limit [Å]	1.920 *
Rmerge	0.038	0.038
Number of reflections	54400 *
Completeness [%]	99.7 *
Redundancy	7.7	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	20-25 *	VAPOR DIFFUSION, HANGING DROP CRYSTALLIZATION CONDITIONS: 1.8M (NH4)2 SO4, 40MM TRIS-HCL PH 7.0, 5MM SPERMINE TRANSFERRED TO 1.9M (NH4)2 SO4, 40MM TRIS-HCL PH 7.0, 5MM SPERMINE, 25% GLYCEROL FOR 15 MINUTES BEFORE FREEZING.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	Tris-HCl	40 (mM)
3	1	reservoir	spermine	5 (mM)