1URI

AZURIN MUTANT WITH MET 121 REPLACED BY GLN

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1991-06-02
Detector	ENRAF-NONIUS FAST
Spacegroup name	C 2 2 21
Unit cell lengths	75.480, 74.340, 98.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.940
R-factor	0.173
Rwork	0.173
RMSD bond length	0.011
RMSD bond angle	2.834
Data reduction software	MADNES
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.940 *	1.940 *
Rmerge	0.077
Total number of observations	58815 *
Number of reflections	16824
Completeness [%]	79.0	27.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	drop	Na, K phosphate	0.1 (M)
3	1	drop	ammonium sulfate	2 (M)
4	1	reservoir	ammonium sulfate	60 (%(w/v)sat)