1UQX
Ralstonia solanacearum lectin (RS-IIL) in complex with alpha-methylmannoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-17 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.132, 61.707, 74.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.170 - 1.700 |
| R-factor | 0.118 |
| Rwork | 0.116 |
| R-free | 0.13900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.661 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.130 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 15416 | |
| <I/σ(I)> | 10.5 | 3.87 |
| Completeness [%] | 96.5 | 86.6 |
| Redundancy | 4.3 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | PEG 1000, TRIS BUFFER, PH 8.2 |
