1UPW

Crystal structure of the human Liver X receptor beta ligand binding domain in complex with a synthetic agonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	137.220, 43.276, 51.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.250
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fcy
RMSD bond length	0.012
RMSD bond angle	1.358
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.121	0.395
Number of reflections	11049
<I/σ(I)>	4.7	1.7
Completeness [%]	89.9	92.4
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		RESERVOIR: 25% MPD, 10% PEG4K, 100 MM HEPES PH 7.5, 10 MM D PROTEIN: 4-8 MG/ML IN 20 MM TRIS PH 8.0, 100 MM NACL, 10 MM