1UPQ
Crystal structure of the pleckstrin homology (PH) domain of PEPP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 93.547, 93.547, 28.786 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.480 |
| R-factor | 0.1809 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eaz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.016 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.500 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.060 | 0.470 |
| Number of reflections | 20221 | |
| <I/σ(I)> | 22 | 2.9 |
| Completeness [%] | 91.7 | 97.5 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG 5000 MME, 0.2 M AMMONIUM SULFATE, 0.1 M MES PH 6.5 |
