1UPK

Crystal structure of MO25 in complex with a C-terminal peptide of STRAD

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-15
Detector	ADSC CCD
Wavelength(s)	0.979355,0.979333
Spacegroup name	P 21 21 2
Unit cell lengths	77.650, 134.033, 40.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 1.850
R-factor	0.202
Rwork	0.202
R-free	0.23100 *
Structure solution method	DIRECT METHODS
RMSD bond length	0.013 *
RMSD bond angle	18.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.073	0.476
Total number of observations	324698 *
Number of reflections	36794
<I/σ(I)>	41.7	4.9
Completeness [%]	99.6	99
Redundancy	8.8	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		15% PEG 20000, 0.1 M MES PH 5.0, 3.3% W/V D-GLUCOSE MONOHYDRATE.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	14.5 (mg/ml)
2	1	reservoir	PEG20000	15 (%(w/v))
3	1	reservoir	MES	0.1 (M)	pH5.0
4	1	reservoir	D-glucose monohydrate	30 (%(w/v))