1UP8
Recombinant vanadium-dependent bromoperoxidase from red algae Corallina pilulifera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 185.948, 185.948, 180.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.000 - 2.200 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qhb |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.123 | 0.403 |
| Number of reflections | 178580 | |
| <I/σ(I)> | 10.5 | 2.3 |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 3.8 * | 0.1M TRIS/H2SO4, 2M NH4H2PO4, pH 4.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris | 50 (mM) | pH7.4 |
| 2 | 1 | drop | citric acid-potassium dihydrogenphosphate | 100 (mM) | pH3.8 |
| 3 | 1 | drop | EDTA | 10 (mM) |
