1UOZ
Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with thiocellopentaose at 1.1 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.940, 92.627, 46.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.300 - 1.100 |
| R-factor | 0.113 |
| Rwork | 0.112 |
| R-free | 0.13000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tml |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.762 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.200 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.075 | 0.123 |
| Number of reflections | 109101 | |
| <I/σ(I)> | 17 | 7.9 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 5.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 14 % PEG 4K, 100 MM HEPES 7.5, 200 MM LISO4. THE PROTEIN WAS AT 10MG/ML AND INCUBATED WITH 1 MM OF THIOCELLOPENTAOSE FOR 1HR PRIOR. CRYSTALLISATION. 20% GLYCEROL WAS ADDED AS CRYOPROTECTANT, pH 7.50 |
