1UNP
Crystal structure of the pleckstrin homology domain of PKB alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.064, 33.803, 42.071 |
| Unit cell angles | 90.00, 119.48, 90.00 |
Refinement procedure
| Resolution | 19.780 - 1.650 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23600 |
| Structure solution method | MAD |
| Starting model (for MR) | 1h10 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.048 | 0.409 |
| Number of reflections | 12409 | |
| <I/σ(I)> | 4.1 | 4.1 |
| Completeness [%] | 98.7 | 97.2 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30 % PEG 4000, 0.25 M SODIUM ACETATE, 0.1 M TRIS PH 8.5 3.3 % POLYPROPYLENE GLYCOL 400 |
