1UNF
The crystal structure of the eukaryotic FeSOD from Vigna unguiculata suggests a new enzymatic mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.985, 48.163, 63.671 |
| Unit cell angles | 90.00, 119.76, 90.00 |
Refinement procedure
| Resolution | 28.770 - 1.970 |
| R-factor | 0.15 |
| Rwork | 0.148 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dt0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.585 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.770 |
| Number of reflections | 20443 | |
| <I/σ(I)> | 6.2 | 0.9 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 200 MM AMMONIUM SULFATE, 100 MM SODIUM ACETATE PH 5.0, 25%(W/V) POLYETHYLENE GLYCOL 4000 |
