1UNA

UNASSEMBLED VIRUS COAT PROTEIN DIMER, BACTERIOPHAGE RNA-BINDING DIMER

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-12-03
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	59.300, 60.500, 67.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
Rwork	0.204
R-free	0.28400
Structure solution method	MOL. REPLACEMENT
Starting model (for MR)	MS2 COAT PROTEIN DIMER (1MS2)
RMSD bond length	0.006
RMSD bond angle	0.031
Data reduction software	XENGEN ((HOWARD)
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	4.790 *	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.074 *	0.123 *
Total number of observations	32352 *
Number of reflections	6248 *	1157 *
<I/σ(I)>	12.9	5
Completeness [%]	94.0	81
Redundancy	3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *	13 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GA dimers	3 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop		50 (mM)
4	1	reservoir	Tris-maleate	0.1 (M)
5	1	reservoir	PEG1000