1UN6
THE CRYSTAL STRUCTURE OF A ZINC FINGER - RNA COMPLEX REVEALS TWO MODES OF MOLECULAR RECOGNITION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.28200, 1.28347, 1.0426 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.598, 191.593, 79.770 |
| Unit cell angles | 90.00, 101.51, 90.00 |
Refinement procedure
| Resolution | 35.190 - 3.100 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.270 * |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.200 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.051 | 0.315 |
| Number of reflections | 15267 | |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 97.9 | 97.5 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | 20% PEG 8000, 200MM KCL, 5MM MGCL2, 50MM MES, PH 5.6, 3MM DTT, 0.3MM ZNSO4 |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | 20% PEG 8000, 200MM KCL, 5MM MGCL2, 50MM MES, PH 5.6, 3MM DTT, 0.3MM ZNSO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 20 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | MES | 50 (mM) | pH5.6 |
| 5 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 6 | 1 | reservoir | 0.3 (mM) | ||
| 7 | 1 | drop | protein | 5 (mg/ml) |
