1UN0

Crystal Structure of Yeast Karyopherin (Importin) alpha in complex with a Nup2p N-terminal fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-21
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	129.813, 140.076, 63.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.21781
Rwork	0.216
R-free	0.25700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ee4
RMSD bond length	0.012 *
RMSD bond angle	1.831 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.086	0.531
Total number of observations	76810 *
Number of reflections	29670	4308 *
<I/σ(I)>	10	1.8
Completeness [%]	97.5	98.3
Redundancy	2.3	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8	18 *	50 MM HEPES PH 6.8, 0.15 M NACL, 24 % PEG3350, 2 % PEG400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	drop	HEPES	50 (mM)	pH6.8
3	1	drop		0.15 (M)
4	1	drop	PEG3350	24 (%)
5	1	drop	PEG400	2 (%)