1UMY
BHMT from rat liver
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.830, 149.270, 96.250 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 48.400 - 2.500 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lt8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.460 |
| Number of reflections | 58532 | |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 16% PEG 1500, PH=8.5, pH 8.50 |
