1UMG
Crystal structure of fructose-1,6-bisphosphatase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9712, 0.9792, 0.9794 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 111.778, 111.778, 153.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.230 - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.19700 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 44654 | |
| <I/σ(I)> | 27.7 | 8.9 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 3.22 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.2 | 298 | PEG 8000, magnesium chloride, sodium chloride, Tris-HCl, fructose-1,6-bisphosphate, pH 8.2, MICROBATCH, temperature 298.0K |
