1UMF
crystal structure of chorismate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 146.478, 146.478, 132.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.130 - 2.250 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 64913 | |
| Completeness [%] | 98.5 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG 400, CaCl2, HEPES, DTT, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
