1UM8

Crystal structure of helicobacter pylori ClpX

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-27
Wavelength(s)	0.9796, 0.9798, 0.9754
Spacegroup name	P 65
Unit cell lengths	78.515, 78.515, 131.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.600
Rwork	0.219
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.185 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.059	0.326
Number of reflections	13273	1369 *
<I/σ(I)>	22.5	2.6
Completeness [%]	93.7	96.3
Redundancy	3.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	Kim, D.Y., (2003) Acta Cryst., D59, 1642. *
1	VAPOR DIFFUSION, HANGING DROP	8	295	Kim, D.Y., (2003) Acta Cryst., D59, 1642. *
1	VAPOR DIFFUSION, HANGING DROP	8	295	Kim, D.Y., (2003) Acta Cryst., D59, 1642. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	imidazole	100 (mM)	pH8.0
3	1	reservoir	ammonium phosphate	1.0 (M)
4	1	reservoir		0.2 (M)