1UM0
Crystal structure of chorismate synthase complexed with FMN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-10 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.9798, 0.9795, 0.95 |
| Spacegroup name | I 4 |
| Unit cell lengths | 146.686, 146.686, 132.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 1.950 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 100703 | |
| Completeness [%] | 99.4 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG 400, CaCl2, HEPES, DTT, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
