1ULV

Crystal Structure of Glucodextranase Complexed with Acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	198.795, 88.247, 80.994
Unit cell angles	90.00, 112.55, 90.00

Refinement procedure

Resolution	49.600 * - 2.420
R-factor	0.196
Rwork	0.196
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	24.800 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400 *	2.510
High resolution limit [Å]	2.420 *	2.420
Rmerge	0.069 *	0.206 *
Total number of observations	184944 *
Number of reflections	48692
Completeness [%]	98.8	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	20 *	PEG 8000, potassium dihydrogen phosphate, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium acetate	20 (mM)	pH6.0
3	1	drop	calcium acetate	5 (mM)
4	1	reservoir	PEG8000	3.0 (%(w/v))
5	1	reservoir	potassium dihydrogen phosphate	80 (mM)
6	1	reservoir	sodium acetate	50 (mM)	pH5.1