1ULU

Crystal structure of tt0143 from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-09
Detector	RIGAKU JUPITER
Wavelength(s)	0.965, 0.9795, 0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	97.033, 96.706, 107.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.200 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.24900
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.087	0.385
Number of reflections	68642
<I/σ(I)>	5.8	1.7
Completeness [%]	99.4	96.4
Redundancy	6.1	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293.0K