1UKY
SUBSTRATE SPECIFICITY AND ASSEMBLY OF CATALYTIC CENTER DERIVED FROM TWO STRUCTURES OF LIGATED URIDYLATE KINASE
Experimental procedure
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.200, 64.200, 185.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.130 |
R-factor | 0.178 |
Rwork | 0.178 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.130 * | 2.130 * |
Total number of observations | 32483 * | |
Number of reflections | 12091 * | |
Completeness [%] | 91.0 * | 64 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | uridylate kinase | 5 (mg/ml) | |
2 | 1 | drop | PEG3350 | 10 (%(w/v)) | |
3 | 1 | drop | n-octyl-beta-D-glucoside | 0.02 (%) | |
4 | 1 | drop | ATP | 1.5-2.0 (mM) | or ADP |
5 | 1 | drop | potassium phosphate | 50 (mM) | |
6 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
7 | 1 | reservoir | potassium phosphate | 50 (mM) |