1UKV
Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9340 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.503, 119.577, 60.723 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.500 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 106322 | |
| <I/σ(I)> | 11.65 | 3.15 |
| Completeness [%] | 98.4 | 96.3 |
| Redundancy | 3.6 | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 16% PEG 8000, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
