1UKT
Crystal structure of Y100L mutant cyclodextrin glucanotransferase compexed with an acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-22 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.680, 74.470, 80.100 |
| Unit cell angles | 85.29, 105.59, 100.81 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.179 |
| Rwork | 0.173 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pam |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 |
| Data reduction software | FFFEAR |
| Data scaling software | FFFEAR |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.770 | 2.050 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.068 | 0.371 |
| Number of reflections | 68926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 293 | Harata, K., (1996) Acta Cryst., D52, 1136. * |
