1UKS
Crystal structure of F183L/F259L mutant cyclodextrin glucanotransferase complexed with a pseudo-maltotetraose derived from acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-05-23 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.850, 74.530, 78.940 |
| Unit cell angles | 85.19, 104.86, 101.04 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.165 |
| Rwork | 0.162 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pam |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.790 |
| Data reduction software | FFFEAR |
| Data scaling software | FFFEAR |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.048 | 0.055 |
| Number of reflections | 102881 | |
| Completeness [%] | 93.2 | 45.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 293 | Harata, K., (1996) Acta Cryst., D52, 1136. * |
