1UKF
Crystal Structure of Pseudomonas Avirulence Protein AvrPphB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9792, 0.9879, 0.9793, 0.9565 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.601, 49.596, 75.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.090 - 1.350 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 22.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.090 | 1.350 |
| High resolution limit [Å] | 1.350 * | 1.300 |
| Rmerge | 0.036 * | 0.232 * |
| Total number of observations | 236773 * | |
| Number of reflections | 39746 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 96.2 | 93.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 293 | Ammonium Sulfate, PIPES, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | TCEP-HCl | 1 (mM) | |
| 4 | 1 | drop | protein | 17 (mg/ml) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.95 (M) | |
| 6 | 1 | reservoir | PIPES | 100 (mM) | pH6.7 |
