1UJZ
Crystal structure of the E7_C/Im7_C complex; a computationally designed interface between the colicin E7 DNase and the Im7 Immunity protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.882, 74.552, 120.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.570 - 2.100 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7cei |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 19513 | |
<I/σ(I)> | 39.8 | |
Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEGMME 2000, ammonium sulfate, Sodium Acetate, Glycerol, DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |