1UII

Crystal structure of Geminin coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-04
Detector	BRANDEIS
Wavelength(s)	0.9782, 0.9783, 0.9789, 0.9611
Spacegroup name	P 21 21 21
Unit cell lengths	37.160, 94.460, 102.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.216
Rwork	0.216
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.900
Data reduction software	DENZO (1.97)
Data scaling software	SCALEPACK (1.97)
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	25309
Completeness [%]	99.7	98.5
Redundancy	6.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	293	0.36M Sodium Acetate, 0.1M TRIS, 30% PEG 4000, pH 8.5, EVAPORATION, temperature 293K
1	EVAPORATION	8.5	293	0.36M Sodium Acetate, 0.1M TRIS, 30% PEG 4000, pH 8.5, EVAPORATION, temperature 293K