1UHO

Crystal structure of Human Phosphodiesterase 5 complexed with Vardenafil(Levitra)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-06-09
Detector	MAC Science DIP-2030
Wavelength(s)	1.12714
Spacegroup name	C 2 2 21
Unit cell lengths	60.523, 156.721, 89.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.940 - 2.500
R-factor	0.197
Rwork	0.197
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.660
High resolution limit [Å]	2.500	2.500
Number of reflections	14204
Completeness [%]	93.5	88.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.2M MgCl2, 8% PEG8000, 100mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K