1UG9

Crystal Structure of Glucodextranase from Arthrobacter globiformis I42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-11
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	199.860, 88.535, 80.781
Unit cell angles	90.00, 113.08, 90.00

Refinement procedure

Resolution	19.900 * - 2.420 *
R-factor	0.19
Rwork	0.189
R-free	0.22700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lf6
RMSD bond length	0.006
RMSD bond angle	24.900 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.590
High resolution limit [Å]	2.420 *	2.420 *
Rmerge	0.070 *	0.223 *
Total number of observations	193427 *
Number of reflections	48376 *
Completeness [%]	96.0 *	62.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6 *	20 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium acetate	20 (mM)	pH6.0
3	1	drop	calcium acetate	5 (mM)
4	1	reservoir	PEG8000	3.0 (%(w/v))
5	1	reservoir	potassium dihydrogen phosphate	80 (mM)
6	1	reservoir	sodium acetate	50 (mM)	pH5.1