1UF2

The Atomic Structure of Rice dwarf Virus (RDV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	285
Detector technology	DIFFRACTOMETER
Collection date	1998-10-14
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	770.000, 795.000, 814.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	228.740 - 3.500
R-factor	0.303
Rwork	0.303
R-free	0.30600
Structure solution method	Molecular Replacement Averaging
Starting model (for MR)	Cryo-electronmicrograph
RMSD bond length	0.010
RMSD bond angle	1.480 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	RAVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	230.000	3.590
High resolution limit [Å]	3.500	3.500
Rmerge	0.186	0.783
Total number of observations	17806888 *
Number of reflections	3001937 *
<I/σ(I)>	3.1	0.9
Completeness [%]	97.7	91.5
Redundancy	5.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Mizuno, H., (1991) J. Mol. Biol., 219, 665. *
1	VAPOR DIFFUSION, HANGING DROP	6	293	Mizuno, H., (1991) J. Mol. Biol., 219, 665. *