1UEG

Crystal structure of amino-terminal microtubule binding domain of EB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-27
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	P 43 21 2
Unit cell lengths	48.589, 48.589, 90.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.880 - 2.400
R-factor	0.223
Rwork	0.223
R-free	0.27900
RMSD bond length	0.016
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.400	2.400
Rmerge	0.109	0.282
Number of reflections	4615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	ammonium sulfate, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K