1UEC
Crystal structure of autoinhibited form of tandem SH3 domain of p47phox
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-09 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 100.217, 100.217, 44.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.990 - 1.820 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.23600 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 300.000 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.050 | 0.274 |
Total number of observations | 124911 * | |
Number of reflections | 21103 | |
<I/σ(I)> | 14.6 | 3.4 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | Yuzawa, S., (2003) Acta Crystallogr., D59, 1479. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25-35 (mg/ml) | |
2 | 1 | drop | sodium fluoride | 50 (mM) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.4-5.6 |
4 | 1 | reservoir | PEG6000 | 16-18 (%) |