1UDR

CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4)

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	53.850, 109.760, 54.000
Unit cell angles	90.00, 111.09, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.189
Rwork	0.189
R-free	0.22200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3chy
RMSD bond length	0.008
RMSD bond angle	1.280
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.077 *
Number of reflections	42474
Completeness [%]	87.2 *	82.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.9 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	ammonium sulfate	1.2 (M)
4	1	reservoir	Tris-HCl	100 (mM)
5	1	reservoir	ammonium sulfate	2.4 (M)