1UDQ

Crystal structure of the tRNA processing enzyme RNase PH T125A mutant from Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-06
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	144.807, 144.807, 83.337
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.228
Rwork	0.228
R-free	0.26300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.481
Number of reflections	23307
<I/σ(I)>	23	2
Completeness [%]	99.4	98.8
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	ammonium sulfate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.0
2	1	drop		5 (mM)
3	1	drop		50 (mM)
4	1	drop		10 (mM)
5	1	drop	protein	1.5 (mg/ml)
6	1	reservoir	ammonium sulfate	2 (M)