1UB3

Crystal Structure of Tetrameric Structure of Aldolase from thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-12-08
Detector	RIGAKU RAXIS V
Wavelength(s)	0.80
Spacegroup name	P 21 21 21
Unit cell lengths	63.820, 96.887, 137.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.250 - 1.400
R-factor	0.203
Rwork	0.202
R-free	0.21100
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.017
RMSD bond angle	0.004
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.490
High resolution limit [Å]	1.400	1.400
Rmerge	0.048
Number of reflections	164987
Completeness [%]	100.0	97.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.9	295	MPD, Tris-HCl, MgCl2, Deoxyribose phosphate , pH 7.9, MICROBATCH, temperature 295.0K