1UAZ
Crystal structure of archaerhodopsin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.100, 128.100, 117.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.400 |
Rwork | 0.230 |
R-free | 0.28190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iw6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | XTALVIEW |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 12632 | |
<I/σ(I)> | 6.5 | 1.3 |
Completeness [%] | 90.4 | 99.9 |
Redundancy | 12.5 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 280 | ammonium sulfate, octylthioglucoside, sodium citrate, heptanetriol, sodium chloride , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 280 | ammonium sulfate, octylthioglucoside, sodium citrate, heptanetriol, sodium chloride , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 280K |