1UAX
Crystal structure of the ribonuclease H2 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-02-01 |
Detector | OXFORD |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.720, 97.650, 159.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.650 - 2.000 |
R-factor | 0.2553 |
Rwork | 0.255 |
R-free | 0.29840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1io2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | OXFORD |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.650 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.126 |
Number of reflections | 55176 | |
<I/σ(I)> | 12.2 | 6.4 |
Completeness [%] | 97.6 | 95.6 |
Redundancy | 3.39 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, 2M Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |