1UA2
Crystal Structure of Human CDK7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.501, 191.635, 75.794 |
| Unit cell angles | 90.00, 94.40, 90.00 |
Refinement procedure
| Resolution | 49.450 - 3.020 |
| R-factor | 0.21659 |
| Rwork | 0.213 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human CDK2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 3.180 |
| High resolution limit [Å] | 2.960 | 3.020 |
| Rmerge | 0.129 | 0.466 |
| Number of reflections | 35416 | |
| <I/σ(I)> | 9.5 | 1.6 |
| Completeness [%] | 92.9 | 70.1 |
| Redundancy | 3.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277 | Na Citrate, Na Acetate, PEG 4000, glycerol, NDSB201, ATP, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 277 | Na Citrate, Na Acetate, PEG 4000, glycerol, NDSB201, ATP, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 4.0K |
